L-S Biotech / Lifesave Biotech Salt-Free MacConkey Agar
(Selective & Differential Medium for Gram-Negative Enteric Bacteria)
Product Description
A specialized salt-free variant of MacConkey Agar designed for superior isolation of Gram-negative enterics while suppressing Proteus swarming.
Key Features
1. Selective Properties
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Bile salts inhibit Gram-positive organisms
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Crystal violet-free formulation (varies by product)
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Salt-free composition prevents Proteus swarming
2. Differential Capability
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Lactose fermentation detection via neutral red indicator:
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Fermenters (e.g., E. coli, Klebsiella): Pink/red colonies
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Non-fermenters (e.g., Salmonella, Pseudomonas): Colorless colonies
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3. Unique Salt-Free Advantage
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Eliminates NaCl to:
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Improve isolation of non-Proteus enterics
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Reduce overgrowth in mixed cultures
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Technical Specifications
| Parameter | Detail |
|---|---|
| Format | Dehydrated powder |
| Selective Agents | Bile salts (crystal violet optional) |
| pH Indicator | Neutral red |
| Key Application | Isolation of enteric bacilli from Proteus-rich samples |
| Regulatory Status | IVD use only |
Applications
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Clinical microbiology (stool/urine cultures)
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Food/water testing
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Research on Proteus-contaminated specimens
Typical Workflow:
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Reconstitute per manufacturer instructions
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Pour plates and inoculate
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Incubate 18-24h at 35±2°C
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Interpret colony morphology/color
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