CLED Agar (Cystine Lactose Electrolyte Deficient Medium)

CLED Agar is a differential, non-selective culture medium designed for isolating, enumerating, and differentiating urinary tract microorganisms.

Key Features:

• Differential Properties:

  • Supports the growth of most urinary pathogens while distinguishing them based on lactose fermentation.

  • Lactose fermenters (e.g., E. coli, Klebsiella) produce yellow colonies due to acid production.

  • Non-lactose fermenters (e.g., Proteus, Pseudomonas) form blue/green colonies.

• Electrolyte Deficiency:

  • Prevents swarming of Proteus, which can overgrow other bacteria in conventional media.

• Composition:

  • L-Cystine: Promotes growth of cysteine-dependent dwarf coliforms.

  • Lactose: Serves as a fermentable carbohydrate source.

  • Bromothymol Blue: pH indicator for colony differentiation.

• Primary Use:

  • Ideal for urine culture (ECBU), especially for quantitative bacterial counts in diagnostic microbiology.

L:S-BIOTECH Nutrient Agar (IVD Grade)

General-Purpose Microbiological Culture Medium
Manufactured/Distributed by LIFESAVE BIOTECH

1. Product Identification

• Product Name: Nutrient Agar

• Brand: L:S-BIOTECH

• Classification:

  • Laboratory reagent (“MORATORY REAGENTS” label)

  • IVD In Vitro Diagnostics (CE marked if applicable)

• Intended Use: Cultivation of non-fastidious microorganisms

2. Key Features

• General-purpose medium for routine bacteriology

• IVD-compliant formulation for diagnostic use

• Standard composition:

  • Peptones (nitrogen source)

  • Beef extract (growth factors)

  • Sodium chloride (osmotic balance)

  • Agar (solidifying agent)

• Typical pH: 7.4 ± 0.2

3. Applications

✔ Quality control testing
✔ Environmental monitoring
✔ Educational demonstrations
✔ Primary isolation of non-fastidious:

• Bacteria

• Yeasts

• Molds

4. Compliance & Safety

• For In Vitro Use Only (Not for human/animal administration)

• Manufactured under ISO 13485 (if IVD-certified)

• Standard shelf life: 3 years when stored sealed at 15-30°C

5. Preparation Protocol

1. Suspend 28g per 1L distilled water

2. Autoclave at 121°C for 15 minutes

3. Pour into sterile Petri dishes

6. Performance Characteristics

• Growth Promotion: Supports growth of:

  • E. coli ATCC 25922

  • S. aureus ATCC 25923

• Sterility: No contamination after incubation

7. Ordering Information

Manufacturer: LIFESAVE BIOTECH
Packaging: Typically 500g bottles
Storage: Room temperature (dry)

L-S Biotech / Lifesave Biotech Salt-Free MacConkey Agar

(Selective & Differential Medium for Gram-Negative Enteric Bacteria)

Product Description

A specialized salt-free variant of MacConkey Agar designed for superior isolation of Gram-negative enterics while suppressing Proteus swarming.

Key Features

1. Selective Properties

• Bile salts inhibit Gram-positive organisms

• Crystal violet-free formulation (varies by product)

• Salt-free composition prevents Proteus swarming

2. Differential Capability

• Lactose fermentation detection via neutral red indicator:

  • Fermenters (e.g., E. coli, Klebsiella): Pink/red colonies

  • Non-fermenters (e.g., Salmonella, Pseudomonas): Colorless colonies

3. Unique Salt-Free Advantage

• Eliminates NaCl to:

  • Improve isolation of non-Proteus enterics

  • Reduce overgrowth in mixed cultures

Technical Specifications

Applications

• Clinical microbiology (stool/urine cultures)

• Food/water testing

• Research on Proteus-contaminated specimens

Typical Workflow:

1. Reconstitute per manufacturer instructions

2. Pour plates and inoculate

3. Incubate 18-24h at 35±2°C

4. Interpret colony morphology/color