Consumables

Hektoen Enteric Agar (HEA) – Technical Specifications

Selective & Differential Medium for Enteric Pathogens

1. Intended Use

• Primary Application: Isolation/differentiation of Salmonella and Shigella spp.

• Sample Types: Clinical stool specimens, food, environmental samples

2. Key Functional Components

3. Physical Characteristics

• Color: Green (prepared medium)

• Clarity: Clear to slightly opalescent

• pH: 7.5 ± 0.2 at 25°C

• Format: Dehydrated powder

Performance Characteristics

Colony Morphology

Quality Control Parameters

• Sterility: No growth after 24h at 35°C

• Growth Promotion:

  • Salmonella enterica ATCC 14028: Blue-green colonies with H₂S

  • Shigella flexneri ATCC 12022: Pale green colonies

• Selectivity: ≥80% inhibition of E. coli ATCC 25922

Protocol Summary

1. Preparation: Suspend 75g/L, boil to dissolve, autoclave (121°C, 15 min)

2. Plating: Pour into Petri dishes (~20mL/plate)

3. Inoculation: Streak for isolation

4. Incubation: 35-37°C for 18-24h (extend to 48h for low-density samples)

Regulatory & Storage

• Shelf Life: 3 years (unopened, <30°C)

• Safety: Handle as potential biohazard post-inoculation

Advantages Over Competing Media

✔ Dual differentiation (fermentation + H₂S)
✔ Superior Shigella recovery vs. SS Agar
✔ Color contrast enhances readability

ReadyMED™ Sabouraud Dextrose Agar with Chloramphenicol (RDM-SDA-02)

Manufactured by Chaitanya Agro Biotech Pvt. Ltd.

Product Overview

A selective fungal culture medium optimized for isolation of yeasts and molds, with added chloramphenicol to suppress bacterial contamination. Ideal for clinical, pharmaceutical, and cosmetic testing.

Key Specifications

1. Composition

2. Selective Properties

• Chloramphenicol (0.05g/L):

  • Broad-spectrum antibiotic

  • Suppresses Gram-positive & Gram-negative bacteria

• Acidic pH (5.6 ± 0.2):

  • Enhances fungal growth

  • Further inhibits bacterial competitors

3. Physical Characteristics

• Form: Free-flowing dehydrated powder

• Prepared Medium: Clear to slightly opaque

• Gel Strength: Firm (1.5% agar equivalent)

Applications

• Clinical Mycology: Isolation of Candida, dermatophytes

• Pharmaceutical QC: Sterility testing (USP/EP compliant)

• Cosmetic Testing: Preservative efficacy evaluation

• Environmental Monitoring: Air and surface fungal sampling

Preparation & Usage

Protocol

1. Suspension: 65g powder per 1L purified water

2. Heating: Boil with stirring until dissolved

3. Sterilization: Autoclave at 121°C for 15 minutes

4. Pouring: Cool to 45-50°C before plating

Incubation

• Yeasts: 30-35°C for 24-48 hours

• Molds: 20-25°C for 3-5 days

Quality & Compliance

• Manufactured under cGMP

• Meets USP/EP/JP standards

• Batch-controlled for consistency

Product Code: RDM-SDA-02
Shelf Life: 36 months (unopened, at 15-30°C)

Why Choose ReadyMED SDA-CAF?

✔ Dual selectivity (antibiotic + acidic pH)
✔ Optimized for fastidious fungi
✔ Regulatory-compliant for critical testing

ReadyMED™ Blood Agar Base (RDM-BAB-02)

Manufactured by Chaitanya Agro Biotech Pvt. Ltd.

Product Overview

A high-quality dehydrated blood agar base medium designed for the cultivation of fastidious microorganisms and hemolysis observation. Ideal for clinical, pharmaceutical, and research laboratories.

Key Features

• Universal base medium for preparing blood agar plates

• Optimal nutrition for fastidious organisms (streptococci, pneumococci, etc.)

• Clear hemolysis visualization when supplemented with 5-10% blood

• Ready-to-prepare dehydrated formulation

Technical Specifications

Applications

• Isolation of pathogenic streptococci

• Hemolysis pattern studies (α, β, γ)

• Routine culture of fastidious organisms

• Clinical microbiology diagnostics

Manufacturer Details

Chaitanya Agro Biotech Pvt. Ltd.

• Registered Corporate Identity Number (CIN) provided on label

• Customer support contacts included

• Website for product information

Preparation Protocol

1. Suspend 40g in 1L distilled water

2. Heat to boiling with stirring

3. Autoclave at 121°C for 15 minutes

4. Cool to 45-50°C; add sterile blood aseptically

5. Pour plates under sterile conditions

Quality Assurance

• Manufactured under strict quality controls

• Batch-to-batch consistency

• Complies with international microbiological standards

L-S Biotech / Lifesave Biotech Salt-Free MacConkey Agar

(Selective & Differential Medium for Gram-Negative Enteric Bacteria)

Product Description

A specialized salt-free variant of MacConkey Agar designed for superior isolation of Gram-negative enterics while suppressing Proteus swarming.

Key Features

1. Selective Properties

• Bile salts inhibit Gram-positive organisms

• Crystal violet-free formulation (varies by product)

• Salt-free composition prevents Proteus swarming

2. Differential Capability

• Lactose fermentation detection via neutral red indicator:

  • Fermenters (e.g., E. coli, Klebsiella): Pink/red colonies

  • Non-fermenters (e.g., Salmonella, Pseudomonas): Colorless colonies

3. Unique Salt-Free Advantage

• Eliminates NaCl to:

  • Improve isolation of non-Proteus enterics

  • Reduce overgrowth in mixed cultures

Technical Specifications

Applications

• Clinical microbiology (stool/urine cultures)

• Food/water testing

• Research on Proteus-contaminated specimens

Typical Workflow:

1. Reconstitute per manufacturer instructions

2. Pour plates and inoculate

3. Incubate 18-24h at 35±2°C

4. Interpret colony morphology/color

ReadyMED Sabouraud Dextrose Agar (RDM-SDA-01) – Technical Specifications

Product Description

ReadyMED Sabouraud Dextrose Agar (SDA) is a selective fungal culture medium optimized for isolation and cultivation of yeasts and molds. The formulation complies with international standards for pharmaceutical and cosmetic testing.

Key Characteristics

1. Composition

2. Selective Properties

• Natural pH 5.6 creates acidic environment that:

  • Promotes fungal growth

  • Inhibits most bacterial contaminants

• Optional antibiotic supplementation (chloramphenicol/gentamicin) available for enhanced selectivity

3. Performance Characteristics

• Growth Promotion: Supports robust development of:

  • Pathogenic yeasts (e.g., Candida albicans)

  • Filamentous fungi

  • Dermatophytes

• Colony Morphology: Produces characteristic fungal colonies for easy identification

Quality Attributes

• Physical Parameters:

  • Dehydrated form: Free-flowing powder

  • Prepared medium: Clear to slightly opalescent

  • Gel strength: Equivalent to 1.5% agar

• pH: 5.6 ± 0.2 at 25°C

Applications

• Pharmaceutical QC:

  • Sterility testing

  • Microbial limit testing

• Clinical Mycology:

  • Isolation of fungal pathogens

• Cosmetic Testing:

  • Preservative efficacy testing

• Environmental Monitoring:

  • Air and surface sampling

Regulatory Status

• Manufactured under cGMP conditions

• Meets compendial requirements (USP/EP)

Product Code: RDM-SDA-01

Packaging: 500g dehydrated powder

Shelf Life: 36 months when stored sealed at 15-30°C

Preparation Protocol

1. Suspend 65g in 1L purified water

2. Heat to boiling with stirring

3. Autoclave at 121°C for 15 minutes

4. Cool to 45-50°C before pouring

Incubation Conditions:

• 20-25°C for 3-5 days (molds)

• 30-35°C for 24-48 hours (yeasts)

ReadyMED Columbia Agar (RDM-CMBA-01) – Product Overview

General Description

ReadyMED Columbia Agar is a highly nutritious, dehydrated culture medium designed for the isolation and cultivation of a broad spectrum of microorganisms, including fastidious organisms such as Streptococci and Pneumococci.

Key Features

1. Primary Applications

• Isolation and cultivation of diverse microorganisms, particularly fastidious bacteria

• Ideal for clinical specimens where broad-spectrum recovery is needed

• Hemolysis detection when supplemented with blood (e.g., 5% sheep blood)

2. Composition & Functionality

3. Preparation

• Form: Dehydrated powder

• Preparation Steps:

  1. Dissolve in distilled water

  2. Boil to ensure complete dissolution

  3. Sterilize by autoclaving

• Optional: Add 5% defibrinated sheep blood for hemolysis studies

4. Quality & Compliance

• Meets harmonized USP/EP/JP pharmacopeial requirements

• Ensures consistent performance for diagnostic and research applications

ReadyMED Eosin Methylene Blue (EMB) Agar – Product Overview

General Description

ReadyMED EMB Agar is a selective and differential dehydrated culture medium designed for the isolation and identification of Gram-negative bacilli (GNB), particularly Enterobacteriaceae.

Key Characteristics

1. Selective & Differential Properties

• Selective for Gram-negative bacteria due to eosin Y and methylene blue dyes, which inhibit most Gram-positive organisms.

• Differential capability distinguishes lactose/sucrose fermenters from non-fermenters based on colony color and morphology.

2. Composition

3. Fermentation Indicators

• Lactose/Sucrose Fermenters:

  • Escherichia coli: Green metallic sheen (characteristic)

  • Other coliforms (e.g., Klebsiella, Enterobacter): Pink to purple colonies

• Non-Fermenters (e.g., Salmonella, Shigella, Pseudomonas): Colorless or pale colonies

Applications

• Water testing: Detection of coliforms (indicator organisms for fecal contamination).

• Food & environmental microbiology: Enumeration of coliforms.

• Clinical bacteriology: Differentiation of Enterobacteriaceae in stool and urine cultures.

Preparation & Usage

• Form: Dehydrated powder (requires reconstitution with distilled water and sterilization).

• ReadyMED Product Code: RDM-EMB-XX (specific code may vary).

ReadyMED Salmonella Shigella Agar (RDM-SSA-01) – Product Description

Appearance:

• Homogeneous, free-flowing powder

• Light yellow to pink in color

Gel Properties:

• Forms a firm gel (equivalent to 1.5% agar)

• Prepared medium appears as a clear to slightly opalescent, reddish-orange gel in Petri dishes

pH:

• 7.0 ± 0.2 (6.3% w/v solution at 25°C)

Composition:

• Selective Agents: Bile salts, sodium citrate, and brilliant green (inhibit Gram-positive bacteria, coliforms, and Proteus spp.)

• Nutrients: Beef extract, enzymatic digest of casein, and animal tissue

• Carbohydrate Source: Lactose

• H₂S Detection: Sodium thiosulfate and ferric citrate

• pH Indicator: Neutral red

Cultural Response (After 18-24 hrs at 35-37°C):

Use:

• Selective isolation of Salmonella and Shigella from clinical, food, and environmental samples

Product Code: RDM-SSA-01

ReadyMED’s Selenite F Broth (Twin Pack) is a dehydrated selective enrichment medium designed for the isolation of Salmonella and Shigella from clinical and non-clinical specimens.

Key Features:

• Medium Type: Selective enrichment broth (Selenite F formulation).

• Purpose: Enhances the growth of Salmonella and Shigella while inhibiting competing flora.

• Packaging: Twin Pack (contains Part-A & Part-B) for convenient medium preparation.

• Manufacturer: Chaitanya Agro Biotech Pvt. Ltd.

• Product Code: RDM-SLF-01

Ideal for microbiological testing in clinical, food, and environmental laboratories.

CLED Agar (Cystine Lactose Electrolyte Deficient Medium)

CLED Agar is a differential, non-selective culture medium designed for isolating, enumerating, and differentiating urinary tract microorganisms.

Key Features:

• Differential Properties:

  • Supports the growth of most urinary pathogens while distinguishing them based on lactose fermentation.

  • Lactose fermenters (e.g., E. coli, Klebsiella) produce yellow colonies due to acid production.

  • Non-lactose fermenters (e.g., Proteus, Pseudomonas) form blue/green colonies.

• Electrolyte Deficiency:

  • Prevents swarming of Proteus, which can overgrow other bacteria in conventional media.

• Composition:

  • L-Cystine: Promotes growth of cysteine-dependent dwarf coliforms.

  • Lactose: Serves as a fermentable carbohydrate source.

  • Bromothymol Blue: pH indicator for colony differentiation.

• Primary Use:

  • Ideal for urine culture (ECBU), especially for quantitative bacterial counts in diagnostic microbiology.

Mannitol Salt Agar (MSA), also called Chapman Agar, is a selective and differential medium used in microbiology to isolate and identify staphylococci, especially Staphylococcus aureus.

Key Characteristics:

Selectivity:

• Contains 7.5% sodium chloride, creating a high-salt environment that inhibits most bacteria except halotolerant staphylococci.

Differentiation:

• Distinguishes staphylococcal species based on mannitol fermentation:

  • Mannitol Fermenters (e.g., S. aureus): Produce acid, turning the phenol red pH indicator from red to yellow around colonies.

  • Non-Fermenters (e.g., S. epidermidis): No acid production; the medium stays red or may turn magenta.

Nutrient Composition:

• Includes peptones and beef extract to support bacterial growth.

Applications:

• Used for isolating pathogenic staphylococci from clinical, food, cosmetic, and water samples.

ReadyMED™ Blood Agar Base (RDM-BAB-02) – Product Specifications

Product Overview

A dehydrated culture medium formulated for preparing blood agar plates and cultivating fastidious microorganisms. Manufactured by Chaitanya Agro Bio-Tech Pvt. Ltd.

Key Features & Applications

Functional Properties

✔ Nutrient-Rich Formula – Supports growth of demanding pathogens
✔ Versatile Usage – Can be used:

• As a base for blood agar (5-10% defibrinated blood addition)

• As a general-purpose medium (without blood)

Primary Applications

• Isolation & Cultivation of Fastidious Bacteria:

  • Streptococcus spp.

  • Neisseria spp.

• Hemolysis Studies:

  • Differentiation of Listeria species

  • CAMP test for Streptococcus agalactiae identification

• Clinical & Diagnostic Use:

  • Blood cultures

  • Respiratory/sputum specimens

Composition Highlights

• Peptones & Yeast Extract – Provide essential nitrogen/vitamin sources

• Sodium Chloride – Maintains osmotic balance

• Agar – Solidifying agent (1.5-2.0% typical concentration)

Technical Specifications

• Form: Free-flowing powder

• pH (after reconstitution): 7.3 ± 0.2

• Sterilization: Autoclave at 121°C for 15 mins

• Storage: Keep sealed at 10-30°C; avoid humidity

Preparation Guide

1. Suspend 40g in 1L distilled water

2. Heat to dissolve completely

3. Sterilize & cool to 45-50°C

4. Add 5-10% sterile blood (if required)

5. Pour into plates

Quality Assurance

• Batch-Tested for microbial growth performance

• ISO Certified manufacturing