Carbol Fuchsin

Carbol Fuchsin is a staining solution used primarily in microbiology, with specific characteristics and uses.

Key Features:
Composition: It is a mixture of phenol and basic fuchsin.
Use in staining: It is essential for staining mycobacteria, particularly in the Ziehl-Neelsen method, due to its affinity for the mycolic acids present in their waxy cell walls.
Resistance to decolorization: Mycobacteria stained with carbol fuchsin are resistant to decolorization by acidic alcohol, allowing them to be distinguished from other bacteria.
Microscopic Appearance: Acid-fast bacteria appear red or magenta-red under the microscope after staining with carbol fuchsin. Stability: The solution should be stored in an airtight, light-resistant container at a temperature below 40°C, preferably between 15 and 30°C, avoiding freezing.

Main Applications:
Disease Diagnosis: Crucial in the diagnosis of diseases such as tuberculosis and leprosy, as it helps identify the responsible acid-fast bacteria.
Microbiology Research: Used for the microscopic study of acid-fast bacteria and the staining of bacterial spores.
Topical Medical Use: Can be used as a first aid antiseptic for certain skin conditions where there is excess moisture.