ReadyMED Eosin Methylene Blue (EMB) Agar – Product Overview
General Description
ReadyMED EMB Agar is a selective and differential dehydrated culture medium designed for the isolation and identification of Gram-negative bacilli (GNB), particularly Enterobacteriaceae.
Key Characteristics
1. Selective & Differential Properties
-
Selective for Gram-negative bacteria due to eosin Y and methylene blue dyes, which inhibit most Gram-positive organisms.
-
Differential capability distinguishes lactose/sucrose fermenters from non-fermenters based on colony color and morphology.
2. Composition
| Component | Function |
|---|---|
| Peptone | Nutrient source |
| Lactose & Sucrose | Fermentable carbohydrates |
| Dipotassium phosphate | pH buffer |
| Agar | Gelling agent |
| Eosin Y & Methylene Blue | – Selective inhibitors – pH indicators (color change on acidification) |
3. Fermentation Indicators
-
Lactose/Sucrose Fermenters:
-
Escherichia coli: Green metallic sheen (characteristic)
-
Other coliforms (e.g., Klebsiella, Enterobacter): Pink to purple colonies
-
-
Non-Fermenters (e.g., Salmonella, Shigella, Pseudomonas): Colorless or pale colonies
Applications
-
Water testing: Detection of coliforms (indicator organisms for fecal contamination).
-
Food & environmental microbiology: Enumeration of coliforms.
-
Clinical bacteriology: Differentiation of Enterobacteriaceae in stool and urine cultures.
Preparation & Usage
-
Form: Dehydrated powder (requires reconstitution with distilled water and sterilization).
-
ReadyMED Product Code: RDM-EMB-XX (specific code may vary).
Reviews
There are no reviews yet.