Müller Hinton Agar

ReadyMED™ Mueller Hinton Agar (RDM-MHA-01)

Standardized Medium for Antimicrobial Susceptibility Testing
Manufactured by Chaitanya Agro Biotech Pvt. Ltd. (Buldhana, Maharashtra, India)

1. Product Overview

• Form: White biotech-grade dehydrated powder

• Packaging: 1 kg bottles

• Storage: 2-8°C (until expiration date)

• Purity: ≥95%

2. Key Applications

• Kirby-Bauer disk diffusion testing

• Broth microdilution assays

• Standardized AST for non-fastidious pathogens

3. Composition & Performance

Critical Properties:

• Low levels of sulfonamide/trimethoprim inhibitors

• pH 7.3 ± 0.1 (after solidification)

• 4mm depth compliance for disk diffusion

4. Quality Assurance

• Batch-to-batch consistency for reproducible zone sizes

• Validated with reference strains:

  • E. coli ATCC 25922

  • S. aureus ATCC 29213

  • P. aeruginosa ATCC 27853

• Meets CLSI/EUCAST standards

5. Preparation Protocol

1. Suspension: 38g per 1L distilled water

2. Heating: Boil with stirring (100°C, 1 min)

3. Sterilization: Autoclave (121°C, 15 min)

4. Pouring: 25mL per 90mm plate (4mm depth)

5. QC Testing: Verify with control strains

6. Manufacturer Credentials

• Parent Company: Chaitanya Group

• Facility: cGMP-compliant production

• Regulatory Status: IVD-grade (CE marked if applicable)

7. Why Choose ReadyMED MHA?

✔ CLSI-compliant formulation
✔ Optimized for accurate zone edge definition
✔ Traceable raw materials
✔ Long shelf life (typically 3 years)

Product Code: RDM-MHA-01