{"id":17522,"date":"2025-08-05T16:08:25","date_gmt":"2025-08-05T16:08:25","guid":{"rendered":"https:\/\/orickmedicosarl.com\/?post_type=product&p=17522"},"modified":"2025-08-11T17:02:28","modified_gmt":"2025-08-11T17:02:28","slug":"eosin-methylene-blue-agar","status":"publish","type":"product","link":"https:\/\/orickmedicosarl.com\/index.php\/product\/eosin-methylene-blue-agar\/","title":{"rendered":"Eosin Methylene Blue Agar"},"content":{"rendered":"

ReadyMED Eosin Methylene Blue (EMB) Agar \u2013 Product Overview<\/strong><\/h3>\n

General Description<\/strong><\/h4>\n

ReadyMED EMB Agar is a\u00a0selective and differential dehydrated culture medium<\/strong>\u00a0designed for the isolation and identification of\u00a0Gram-negative bacilli (GNB)<\/strong>, particularly\u00a0Enterobacteriaceae<\/em>.<\/p>\n

\n

Key Characteristics<\/strong><\/h3>\n

1. Selective & Differential Properties<\/strong><\/h4>\n
    \n
  • \n

    Selective for Gram-negative bacteria<\/strong>\u00a0due to eosin Y and methylene blue dyes, which inhibit most Gram-positive organisms.<\/p>\n<\/li>\n

  • \n

    Differential capability<\/strong>\u00a0distinguishes lactose\/sucrose fermenters from non-fermenters based on colony color and morphology.<\/p>\n<\/li>\n<\/ul>\n

    2. Composition<\/strong><\/h4>\n
    \n\n\n\n\n\n\n\n\n\n
    Component<\/th>\nFunction<\/th>\n<\/tr>\n<\/thead>\n
    Peptone<\/td>\nNutrient source<\/td>\n<\/tr>\n
    Lactose & Sucrose<\/td>\nFermentable carbohydrates<\/td>\n<\/tr>\n
    Dipotassium phosphate<\/td>\npH buffer<\/td>\n<\/tr>\n
    Agar<\/td>\nGelling agent<\/td>\n<\/tr>\n
    Eosin Y & Methylene Blue<\/strong><\/td>\n– Selective inhibitors
    \n– pH indicators (color change on acidification)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    3. Fermentation Indicators<\/strong><\/h4>\n
      \n
    • \n

      Lactose\/Sucrose Fermenters<\/strong>:<\/p>\n

        \n
      • \n

        Escherichia coli<\/em>:\u00a0Green metallic sheen<\/strong>\u00a0(characteristic)<\/p>\n<\/li>\n

      • \n

        Other coliforms (e.g.,\u00a0Klebsiella<\/em>,\u00a0Enterobacter<\/em>):\u00a0Pink to purple colonies<\/strong><\/p>\n<\/li>\n<\/ul>\n<\/li>\n

      • \n

        Non-Fermenters<\/strong>\u00a0(e.g.,\u00a0Salmonella<\/em>,\u00a0Shigella<\/em>,\u00a0Pseudomonas<\/em>):\u00a0Colorless or pale colonies<\/strong><\/p>\n<\/li>\n<\/ul>\n

        \n

        Applications<\/strong><\/h3>\n
          \n
        • \n

          Water testing<\/strong>: Detection of coliforms (indicator organisms for fecal contamination).<\/p>\n<\/li>\n

        • \n

          Food & environmental microbiology<\/strong>: Enumeration of coliforms.<\/p>\n<\/li>\n

        • \n

          Clinical bacteriology<\/strong>: Differentiation of\u00a0Enterobacteriaceae<\/em>\u00a0in stool and urine cultures.<\/p>\n<\/li>\n<\/ul>\n

          \n

          Preparation & Usage<\/strong><\/h3>\n
            \n
          • \n

            Form<\/strong>: Dehydrated powder (requires reconstitution with distilled water and sterilization).<\/p>\n<\/li>\n

          • \n

            ReadyMED Product Code<\/strong>:\u00a0RDM-EMB-XX<\/strong>\u00a0(specific code may vary).<\/p>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

            ReadyMED Eosin Methylene Blue (EMB) Agar \u2013 Product Overview<\/strong><\/h3>\n

            General Description<\/strong><\/h4>\n

            ReadyMED EMB Agar is a\u00a0selective and differential dehydrated culture medium<\/strong>\u00a0designed for the isolation and identification of\u00a0Gram-negative bacilli (GNB)<\/strong>, particularly\u00a0Enterobacteriaceae<\/em>.<\/p>\n

            \n

            Key Characteristics<\/strong><\/h3>\n

            1. Selective & Differential Properties<\/strong><\/h4>\n
              \n
            • \n

              Selective for Gram-negative bacteria<\/strong>\u00a0due to eosin Y and methylene blue dyes, which inhibit most Gram-positive organisms.<\/p>\n<\/li>\n

            • \n

              Differential capability<\/strong>\u00a0distinguishes lactose\/sucrose fermenters from non-fermenters based on colony color and morphology.<\/p>\n<\/li>\n<\/ul>\n

              2. Composition<\/strong><\/h4>\n
              \n\n\n\n\n\n\n\n\n\n
              Component<\/th>\nFunction<\/th>\n<\/tr>\n<\/thead>\n
              Peptone<\/td>\nNutrient source<\/td>\n<\/tr>\n
              Lactose & Sucrose<\/td>\nFermentable carbohydrates<\/td>\n<\/tr>\n
              Dipotassium phosphate<\/td>\npH buffer<\/td>\n<\/tr>\n
              Agar<\/td>\nGelling agent<\/td>\n<\/tr>\n
              Eosin Y & Methylene Blue<\/strong><\/td>\n– Selective inhibitors
              \n– pH indicators (color change on acidification)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

              3. Fermentation Indicators<\/strong><\/h4>\n
                \n
              • \n

                Lactose\/Sucrose Fermenters<\/strong>:<\/p>\n

                  \n
                • \n

                  Escherichia coli<\/em>:\u00a0Green metallic sheen<\/strong>\u00a0(characteristic)<\/p>\n<\/li>\n

                • \n

                  Other coliforms (e.g.,\u00a0Klebsiella<\/em>,\u00a0Enterobacter<\/em>):\u00a0Pink to purple colonies<\/strong><\/p>\n<\/li>\n<\/ul>\n<\/li>\n

                • \n

                  Non-Fermenters<\/strong>\u00a0(e.g.,\u00a0Salmonella<\/em>,\u00a0Shigella<\/em>,\u00a0Pseudomonas<\/em>):\u00a0Colorless or pale colonies<\/strong><\/p>\n<\/li>\n<\/ul>\n

                  \n

                  Applications<\/strong><\/h3>\n
                    \n
                  • \n

                    Water testing<\/strong>: Detection of coliforms (indicator organisms for fecal contamination).<\/p>\n<\/li>\n

                  • \n

                    Food & environmental microbiology<\/strong>: Enumeration of coliforms.<\/p>\n<\/li>\n

                  • \n

                    Clinical bacteriology<\/strong>: Differentiation of\u00a0Enterobacteriaceae<\/em>\u00a0in stool and urine cultures.<\/p>\n<\/li>\n<\/ul>\n

                    \n

                    Preparation & Usage<\/strong><\/h3>\n
                      \n
                    • \n

                      Form<\/strong>: Dehydrated powder (requires reconstitution with distilled water and sterilization).<\/p>\n<\/li>\n

                    • \n

                      ReadyMED Product Code<\/strong>:\u00a0RDM-EMB-XX<\/strong>\u00a0(specific code may vary).<\/p>\n<\/li>\n<\/ul>\n","protected":false},"featured_media":17523,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[203],"product_cat":[167],"product_tag":[],"class_list":{"0":"post-17522","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-readymed","7":"product_cat-consumables","9":"first","10":"instock","11":"taxable","12":"shipping-taxable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17522","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/comments?post=17522"}],"version-history":[{"count":1,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17522\/revisions"}],"predecessor-version":[{"id":17524,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17522\/revisions\/17524"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/media\/17523"}],"wp:attachment":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/media?parent=17522"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=17522"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=17522"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=17522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}