{"id":17540,"date":"2025-08-05T16:22:41","date_gmt":"2025-08-05T16:22:41","guid":{"rendered":"https:\/\/orickmedicosarl.com\/?post_type=product&p=17540"},"modified":"2025-08-11T17:03:41","modified_gmt":"2025-08-11T17:03:41","slug":"hektoen-enteric-agar","status":"publish","type":"product","link":"https:\/\/orickmedicosarl.com\/index.php\/product\/hektoen-enteric-agar\/","title":{"rendered":"Hektoen Enteric Agar"},"content":{"rendered":"

Hektoen Enteric Agar (HEA) \u2013 Technical Specifications<\/strong><\/h3>\n

Selective & Differential Medium for Enteric Pathogens<\/em><\/p>\n

\n

1. Intended Use<\/strong><\/h4>\n
    \n
  • \n

    Primary Application<\/strong>: Isolation\/differentiation of\u00a0Salmonella<\/em>\u00a0and\u00a0Shigella<\/em>\u00a0spp.<\/p>\n<\/li>\n

  • \n

    Sample Types<\/strong>: Clinical stool specimens, food, environmental samples<\/p>\n<\/li>\n<\/ul>\n

    2. Key Functional Components<\/strong><\/h4>\n
    \n\n\n\n\n\n\n\n\n
    Component<\/strong><\/th>\nRole<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    Bile salts<\/td>\nInhibits Gram-positive & non-pathogenic Gram-negative bacteria<\/td>\n<\/tr>\n
    Lactose\/sucrose\/salicin<\/td>\nCarbohydrate sources for fermentation<\/td>\n<\/tr>\n
    Sodium thiosulfate + Ferric ammonium citrate<\/td>\nH\u2082S production detection<\/td>\n<\/tr>\n
    Bromothymol blue + Acid fuchsin<\/td>\npH indicators (differentiate fermenters)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    3. Physical Characteristics<\/strong><\/h4>\n
      \n
    • \n

      Color<\/strong>: Green (prepared medium)<\/p>\n<\/li>\n

    • \n

      Clarity<\/strong>: Clear to slightly opalescent<\/p>\n<\/li>\n

    • \n

      pH<\/strong>: 7.5 \u00b1 0.2 at 25\u00b0C<\/p>\n<\/li>\n

    • \n

      Format<\/strong>: Dehydrated powder<\/p>\n<\/li>\n<\/ul>\n

      \n

      Performance Characteristics<\/strong><\/h3>\n

      Colony Morphology<\/strong><\/h4>\n
      \n\n\n\n\n\n\n\n\n
      Organism<\/strong><\/th>\nColony Appearance<\/strong><\/th>\nBiochemical Basis<\/strong><\/th>\n<\/tr>\n<\/thead>\n
      E. coli<\/em>\u00a0(coliform)<\/td>\nSalmon-pink, may yellow medium<\/td>\nRapid lactose fermentation<\/td>\n<\/tr>\n
      Salmonella<\/em>\u00a0spp.<\/td>\nBlue-green with black centers<\/td>\nH\u2082S production (thiosulfate \u2192 FeS)<\/td>\n<\/tr>\n
      Shigella<\/em>\u00a0spp.<\/td>\nPale green, translucent<\/td>\nNon-lactose fermenter<\/td>\n<\/tr>\n
      Proteus<\/em>\u00a0spp.<\/td>\nColorless with blackening<\/td>\nH\u2082S production only<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
      \n

      Quality Control Parameters<\/strong><\/h3>\n
        \n
      • \n

        Sterility<\/strong>: No growth after 24h at 35\u00b0C<\/p>\n<\/li>\n

      • \n

        Growth Promotion<\/strong>:<\/p>\n

          \n
        • \n

          Salmonella enterica<\/em>\u00a0ATCC 14028: Blue-green colonies with H\u2082S<\/p>\n<\/li>\n

        • \n

          Shigella flexneri<\/em>\u00a0ATCC 12022: Pale green colonies<\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          Selectivity<\/strong>: \u226580% inhibition of\u00a0E. coli<\/em>\u00a0ATCC 25922<\/p>\n<\/li>\n<\/ul>\n

          \n

          Protocol Summary<\/strong><\/h3>\n
            \n
          1. \n

            Preparation<\/strong>: Suspend 75g\/L, boil to dissolve, autoclave (121\u00b0C, 15 min)<\/p>\n<\/li>\n

          2. \n

            Plating<\/strong>: Pour into Petri dishes (~20mL\/plate)<\/p>\n<\/li>\n

          3. \n

            Inoculation<\/strong>: Streak for isolation<\/p>\n<\/li>\n

          4. \n

            Incubation<\/strong>: 35-37\u00b0C for 18-24h (extend to 48h for low-density samples)<\/p>\n<\/li>\n<\/ol>\n

            \n

            Regulatory & Storage<\/strong><\/h3>\n
              \n
            • \n

              Shelf Life<\/strong>: 3 years (unopened, <30\u00b0C)<\/p>\n<\/li>\n

            • \n

              Safety<\/strong>: Handle as potential biohazard post-inoculation<\/p>\n<\/li>\n<\/ul>\n

              \n

              Advantages Over Competing Media<\/strong><\/h3>\n

              \u2714\u00a0Dual differentiation<\/strong>\u00a0(fermentation + H\u2082S)
              \n\u2714\u00a0Superior\u00a0Shigella<\/em>\u00a0recovery<\/strong>\u00a0vs. SS Agar
              \n\u2714\u00a0Color contrast<\/strong>\u00a0enhances readability<\/p>\n","protected":false},"excerpt":{"rendered":"

              Hektoen Enteric Agar (HEA) \u2013 Technical Specifications<\/strong><\/h3>\n

              Selective & Differential Medium for Enteric Pathogens<\/em><\/p>\n

              \n

              1. Intended Use<\/strong><\/h4>\n
                \n
              • \n

                Primary Application<\/strong>: Isolation\/differentiation of\u00a0Salmonella<\/em>\u00a0and\u00a0Shigella<\/em>\u00a0spp.<\/p>\n<\/li>\n

              • \n

                Sample Types<\/strong>: Clinical stool specimens, food, environmental samples<\/p>\n<\/li>\n<\/ul>\n

                2. Key Functional Components<\/strong><\/h4>\n
                \n\n\n\n\n\n\n\n\n
                Component<\/strong><\/th>\nRole<\/strong><\/th>\n<\/tr>\n<\/thead>\n
                Bile salts<\/td>\nInhibits Gram-positive & non-pathogenic Gram-negative bacteria<\/td>\n<\/tr>\n
                Lactose\/sucrose\/salicin<\/td>\nCarbohydrate sources for fermentation<\/td>\n<\/tr>\n
                Sodium thiosulfate + Ferric ammonium citrate<\/td>\nH\u2082S production detection<\/td>\n<\/tr>\n
                Bromothymol blue + Acid fuchsin<\/td>\npH indicators (differentiate fermenters)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

                3. Physical Characteristics<\/strong><\/h4>\n
                  \n
                • \n

                  Color<\/strong>: Green (prepared medium)<\/p>\n<\/li>\n

                • \n

                  Clarity<\/strong>: Clear to slightly opalescent<\/p>\n<\/li>\n

                • \n

                  pH<\/strong>: 7.5 \u00b1 0.2 at 25\u00b0C<\/p>\n<\/li>\n

                • \n

                  Format<\/strong>: Dehydrated powder<\/p>\n<\/li>\n<\/ul>\n

                  \n

                  Performance Characteristics<\/strong><\/h3>\n

                  Colony Morphology<\/strong><\/h4>\n
                  \n\n\n\n\n\n\n\n\n
                  Organism<\/strong><\/th>\nColony Appearance<\/strong><\/th>\nBiochemical Basis<\/strong><\/th>\n<\/tr>\n<\/thead>\n
                  E. coli<\/em>\u00a0(coliform)<\/td>\nSalmon-pink, may yellow medium<\/td>\nRapid lactose fermentation<\/td>\n<\/tr>\n
                  Salmonella<\/em>\u00a0spp.<\/td>\nBlue-green with black centers<\/td>\nH\u2082S production (thiosulfate \u2192 FeS)<\/td>\n<\/tr>\n
                  Shigella<\/em>\u00a0spp.<\/td>\nPale green, translucent<\/td>\nNon-lactose fermenter<\/td>\n<\/tr>\n
                  Proteus<\/em>\u00a0spp.<\/td>\nColorless with blackening<\/td>\nH\u2082S production only<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
                  \n

                  Quality Control Parameters<\/strong><\/h3>\n
                    \n
                  • \n

                    Sterility<\/strong>: No growth after 24h at 35\u00b0C<\/p>\n<\/li>\n

                  • \n

                    Growth Promotion<\/strong>:<\/p>\n

                      \n
                    • \n

                      Salmonella enterica<\/em>\u00a0ATCC 14028: Blue-green colonies with H\u2082S<\/p>\n<\/li>\n

                    • \n

                      Shigella flexneri<\/em>\u00a0ATCC 12022: Pale green colonies<\/p>\n<\/li>\n<\/ul>\n<\/li>\n

                    • \n

                      Selectivity<\/strong>: \u226580% inhibition of\u00a0E. coli<\/em>\u00a0ATCC 25922<\/p>\n<\/li>\n<\/ul>\n

                      \n

                      Protocol Summary<\/strong><\/h3>\n
                        \n
                      1. \n

                        Preparation<\/strong>: Suspend 75g\/L, boil to dissolve, autoclave (121\u00b0C, 15 min)<\/p>\n<\/li>\n

                      2. \n

                        Plating<\/strong>: Pour into Petri dishes (~20mL\/plate)<\/p>\n<\/li>\n

                      3. \n

                        Inoculation<\/strong>: Streak for isolation<\/p>\n<\/li>\n

                      4. \n

                        Incubation<\/strong>: 35-37\u00b0C for 18-24h (extend to 48h for low-density samples)<\/p>\n<\/li>\n<\/ol>\n

                        \n

                        Regulatory & Storage<\/strong><\/h3>\n
                          \n
                        • \n

                          Shelf Life<\/strong>: 3 years (unopened, <30\u00b0C)<\/p>\n<\/li>\n

                        • \n

                          Safety<\/strong>: Handle as potential biohazard post-inoculation<\/p>\n<\/li>\n<\/ul>\n

                          \n

                          Advantages Over Competing Media<\/strong><\/h3>\n

                          \u2714\u00a0Dual differentiation<\/strong>\u00a0(fermentation + H\u2082S)
                          \n\u2714\u00a0Superior\u00a0Shigella<\/em>\u00a0recovery<\/strong>\u00a0vs. SS Agar
                          \n\u2714\u00a0Color contrast<\/strong>\u00a0enhances readability<\/p>\n","protected":false},"featured_media":17541,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[203],"product_cat":[167],"product_tag":[],"class_list":{"0":"post-17540","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-readymed","7":"product_cat-consumables","9":"first","10":"instock","11":"taxable","12":"shipping-taxable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17540","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/comments?post=17540"}],"version-history":[{"count":1,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17540\/revisions"}],"predecessor-version":[{"id":17542,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product\/17540\/revisions\/17542"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/media\/17541"}],"wp:attachment":[{"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/media?parent=17540"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=17540"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=17540"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/orickmedicosarl.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=17540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}